The Gadsden flag, proposing a rattlesnake and the word "Don't Tread on Me

https://archerdwgn450.yousher.com/the-craftsmanship-behind-historic-civil-war-flags-techniques-and-materials-used

The Gadsden flag, offering a rattlesnake and the phrase "Don't Tread on Me," became followed by way of some Confederate sets as a symbol of resistance against perceived oppression.