The Gadsden flag, that includes a rattlesnake and the word "Don't Tread on Me

https://landenxuhs760.quantlynix.com/posts/reenacting-the-past-the-significance-of-using-authentic-civil-war-flags

The Gadsden flag, featuring a rattlesnake and the word "Don't Tread on Me," become adopted by some Confederate instruments as a symbol of resistance opposed to perceived oppression.